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. 2021 Jan 26;20:551–558. doi: 10.1016/j.omtm.2021.01.012

Figure 3.

Figure 3

The mechanism of ABCA1 overexpression on ANXA1 nuclear localization

(A) Western blot analysis showing the expression of ANXA1 in the cellular nuclear extract in HEK293 cells. (B) Statistical analysis of (A). ∗∗p < 0.01, compared with control; ##p < 0.01 compared with the LPS group. (C) A western blotting analysis indicates that LPS induces the interaction between importin β and ANXA1, and this effect is inhibited by Ad-ABCA1 treatment. IP, immunoprecipitation; IB, immunoblotting; and WB, western blot. (D) A western blotting analysis indicates that ABCA1 siRNA induces the interaction between importin β and ANXA1. (E) Western blot analysis showing the expression of ANXA1 in the cellular nuclear extract in HEK293 cells. IPZ, importazole. (F) Statistical analysis of (E). ∗p < 0.05, compared with controls; #p < 0.05 compared with the ABCA1 siRNA group. All of the data of the three independent experiments are expressed as the mean ± SD, one-way ANOVA with Bonferroni post hoc test.