Table 1.

Primer sets for amplification of the whole genome of HMPV

Target genes	Primers	Primer sequence (5′–3′)	Location (nt)a	Annealing temp (°C)
N	N(F)	GCGAAAAAAACGCGTATA	3–20	50
	N(R)	TTGCTGCTTCATTACCCAT	1296–1314
P	P(F)	GAAGAAAAAGARGCTGCAGA	1178–1196	50
	P(R)	CCAGATCAACTTGAACAGC	2228–2246
M	M(F)	GAATCMGAAGAAGAAGAAG	2070–2088	50
	M(R)	CAYCACTTTCCAAGACATT	3066–3084
F	F-1(F)	CAATCMAAAAGGYATATTC	2800–2818	50.2
	F-1(R)	CTGCAGATGTYGGCATGT	3764–3781
	F-2(F)	CAGTCAATTCAACAGAAG	3645–3662	50
	F-2(R)	TTCATATTTGCATGGAGC	4736–4753
M2	M2(F)	CCTCCAGAGCTGARTGGTGT	4636–4655	50
	M2(R)	TWTCATTGTCAYTTATCCCA	5467–5486
SH	SH(F)	GCAAGACAGTGAAAGCAYT	5254–5272	46.8
	SH(R)	CATCYTTGCTTTGARCAT	6268–6285
G	G(F)	AAACAARAAWATGGGACAAG	6207–6226	50
	G(R)	TAGACATTRACAGTRGAYTC	7148–7167
L	L-1(F)	GACAAATRRCAATGGATC	7122–7139	44.7
	L-1(R)	TAGTTCTGTYAARCTCTC	8303–8320
	L-2(F)	GARAATGCTGCWGAATTA	8186–8203	46.8
	L-2(R)	GATATHGCYTCCATTGTCC	9288–9306
	L-3(F)	ACATGCACCACCAGAAAC	9178–9195	46.8
	L-3(R)	AGGTGTTATGTTKTCWGC	10,238–10,225
	L-4(F)	GTGAYATMAATAGAACAGC	10,128–10,146	43.8
	L-4(R)	ACTGAAGATRTGTTGATC	11,231–11,248
	L-5(F)	GGAATTAGYATAATGAGTG	11,081–11,099	48
	L-5(R)	GGATATTCACATGCTGTTC	12,246–12,264
	L-6(F)	CTAACAAGRAATTACATG	12,071–12,088	48
	L-6(R)	ACGGCAAAAAAACCGTAT	13,318–13,335

^aThe reference of location is based on AY297749 strain