Figure 4.

AT1-β-arrestin-dependent internalization of membrane LOX-1 in response to oxLDL treatment

(A) Calculation of change in red puncta of LOX-1. Real-time membrane imaging of CHO-K1 cells co-transfected with LOX-1-mScarlet and control, AT1, AT1mβ, or AT1mg labeled with eGFP (Video S1). A count of puncta was performed using separated images visualizing LOX-1-scarlet just before and 3 min after ligand application.

(B) Example of counting red puncta in CHO-K1 cells co-expressing LOX-1-scarlet and AT1-GFP applied with oxLDL. In this case, the change in LOX-1 puncta is (228 − 188)/288 × 100 = 17.5%.

(C) Change in LOX-1 puncta in CHO-K1 cells expressing the indicated receptors (n = 6–9).

Data are represented as mean ± SEM. The difference between the application of vehicle and oxLDL was determined by Student's t-test. ∗p < 0.01.