Figure EV4. Functional validation of reactive lysine in G6PD.

1. Shows CADD38 max codon missense scores for residues 1–350 of G6PD (UniProt ID P11413). CpD K205 has the highest score out of all positions in protein. CpDAA positions above CADD38 deleterious threshold (gray dash line) include K47, K89, C158, K171, K205, and C294.
2. Crystal structure of G6PD (PDB ID: 2BH9) shows K205 and K171 located within the enzyme active site. NADP⁺ cofactor shown in yellow. Surface colored by CADD38 max codon missense scores. Image generated in PyMOL (Smith et al, 2019).