Figure EV5. 2019 Cysteine chemoproteomics data support residue reactivity and deleteriousness score trend.

• A, B
  Association between cysteine reactivity labels and CADD38 (model for GRCh38) PHRED scores for cysteines of low (n = 2,247), medium (n = 1,448), and high (n = 322) intrinsic reactivities, defined by isoTOP‐ABPP ratios, low (R _10:1 > 5), medium (2 < R _10:1 < 5), high (R _10:1 < 2) (Weerapana et al, 2010; Hacker et al, 2017). Either the max CADD score for a missense change was assigned to the codon (BH‐adjusted P‐values, low vs med ***P. adj = 0.00099, low vs high ***P. adj = 0.00086) (A) or the average of all missense scores at that codon (BH‐adjusted P‐values, low vs med ***P. adj = 4.0e‐04, med vs high *P. adj = 0.023, low vs high ****P. adj = 3.90e‐05) (B). Reactivity group differences assessed by Kruskal–Wallis nonparametric test and Wilcox test used for pairwise comparisons.
• C
  Plot of cysteine reactivity ratios for 3,590 out of 4,017 total profiled residues in 2019 isoTOP‐ABPP study. Represented are 322 high, 1,448 medium, and 1,820 low threshold cysteines.