Table 2.
Reagents and other materials
| Product | Vendor | Catalog no. | RRID |
|---|---|---|---|
| Neg-50 | ThermoScientific | 6502 | |
| Fluoromount G | SouthernBiotech | 0100-01 | |
| DAPI | Sigma-Aldrich | D9542-1MG | |
| DMEM | Corning | 10013-CV | |
| Phenol red-free DMEM | Life technologies | 31053-028 | |
| DMEM/F12 | Gibco | 11039-021 | |
| Mouse M-CSF | Shenandoah | 200-08 | |
| Percoll | GE Healthcare | 17-0891-01 | |
| Papain | Sigma | P3125 | |
| Dispase II | Stemcell | 07913 | |
| DNase I | Sigma | D4263 | |
| Bovine serum albumin | Sigma | A7030 | |
| Fetal bovine aerum | Atlanta Biologicalalals | S11150H | |
| Penicillin/streptomycin | Cellgro | 30-008-CI | |
| Poly-D-lysine | Sigma | P6407-5MG | |
| PowerUP SYBR Green Master Mix | ThermoFisher | A25777 | |
| TRIzol | Life Technologies | 15596018 | |
| Verso cDNA synthesis kit | ThermoFisher | AB1453B | |
| Pierce BCA Protein Assay kit | ThermoScientific | 23225 | |
| Chemiluminescent Substrate | ThermoScientific | 34580 | |
| pHrodo Green Zymosan Bioparticles Conjugate for Phagocytosis | ThermoFisher | P35365 | |
| Falcon 40 μm Cell Strainer | Corning | 352340 | |
| Preseparation Filters (30 μm) | Miltenyi | 130-041-407 | |
| MS Columns | Miltenyi | 130-042-201 | |
| Pilocarpine | Sigma | P6503 |