Figure 2.

Sox11 and Sox11^K91A decrease RGC viability and axon outgrowth in vitro. Primary RGCs were transduced with Sox11 or control AAVs as marked, cultured for 3 d, and immunostained for β-III tubulin (E7) and GFP to identified transduced neurons, and counterstained with the nuclear dye DAPI (A). Exogenous expression of either Sox11 or Sox11^K91A reduced cell survival (B) and axon outgrowth (C) in primary RGCs (N ≥ 3 experimental replicates, *p < 0.05, by one-way ANOVA and post hoc t test with Tukey correction; mean ± SEM shown; scale bar: 200 μm).