FIG 1.

Contribution of various flagellins to C. crescentus mobility and phage adsorption. (A) Schematic of phage adsorption to C. crescentus, which includes attachment to the flagella, subsequent interaction with the pilus portal, and genome ejection. (B) Schematic of known organization of flagellin proteins in the C. crescentus flagellum. (C) Cell motility and adsorption kinetics of phage ϕCbK to the wild type and flagellin mutants of C. crescentus. Strain motility and adsorption of phage ϕCbK were measured in triplicate and are normalized relative to the wild-type C. crescentus strain (NA1000). (D) Flagellum length measurements relative to wild-type NA1000 C. crescentus, as determined by negative-stain electron microscopy (Fig. 2). The points indicate individual measurements of each flagellum. Error bars indicate standard deviation, and the central cross bars represent the average length measurement for each strain. For clarity, the strains are named by the flagellin(s) they synthesize.