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Plasmids used in this study
| Plasmid | Description | Reference no. or source |
|---|---|---|
| pMR10 | Broad-host-range cloning vector, Kanr, low copy no.a | 83 |
| pCS1 | Construct with fljK T103C amplified from YB6528 with ∼500 bp of upstream sequence cloned into the BamHI and HindIII sites of pMR10 | This study |
| pCS12 | Construct with fljK T103C N130S; derived from error-prone PCR mutagenesis to pCS1 | This study |
Kanr, resistance to kanamycin.
