FIG 1.

R. capsulatus CckA and CtrA are capable of autophosphorylation, and the phosphorylated proteins are separated from nonphosphorylated proteins in the Phos-tag gel system. (A) Gel migration of CckA versus CckA∼P. (B) Gel migration of CtrA versus CtrA∼P. (C) Mass spectrum of a CckA sample prior to phosphorylation with ATP. (D) Mass spectrum of a CtrA sample prior to phosphorylation with acetyl∼P. (E) Increase in the mass of CckA after incubation with ATP. (F) Increase in the mass of CtrA after incubation with acetyl phosphate.