Fig. 3.

Schematic representation of Balb/c mice immunization with LdHyP and challenge with stationary stage promastigotes of L. donovani to evaluate its prophylactic potential. (A) Study design and Immunization schedule representing the experiments. (B-C) Immunized challenged mice (Cured) showing reduced splenomegaly and hepatomegaly along with no effect on hair loss as compaired to infected mice. (D) Effect of LdHyP in immunized mice body weight, spleen weight and liver weight as compare to infected mice. Significance values represent the difference between cured and infected group of three independent experiments group (n = 4 animals per group). *p < 0.05, **p < 0.01, ns: no significant.