Enzyme linked immunosorbent assays to analyze the LdHyP specific Th1/Th2 cytokines production. Cytokines levels of IFN-γ (A), TNF-α (B), IL-12p40 (C), IL-10 (D) and IL-4 (E) were determined in splenocytes supernatant of all experimental group of mice after 12 week of post challenge. (F) IL-10/IFN-γ ratio. Data represent mean ± SD of two independent experiments in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001, ns: no significant.