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. 2021 Feb 17;20:15. doi: 10.1186/s12944-021-01437-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 12 — a Western blot for Lipin-1 (~ 125 kDa) and GAPDH expression (~ 36 kDa) in non-cancerous prostate cells (PNT2 and RWPE-1), hormone-sensitive (LNCaP and 22RV1), castration-resistant (CR) (PC-3 and DU-145), Docetaxel resistant (DR) (PC3-RX and DU145-DR) prostate cells and hepatocellular carcinoma (HepG2) cells (positive control). Immunoblots are representative of at least 3 (n = 3) separate experiments using 3 separate passages. b Densitometry of Lipin-1 protein normalized to GAPDH within non-cancerous prostate cells (blue), hormone-sensitive (orange), castration-resistant (CR) (green), Docetaxel resistant (DR) (red) prostate, and hepatocellular carcinoma (black) cell lines