Table 2.
Sequences of the primers used for qPCR validation of RNA-Seq data
| Gene name | Primer sequence (5′ to 3′) | Amplicon size (bp) |
|---|---|---|
| Beta-actin | Forward: TGTCACCAACTGGGACGATA | 165 |
| Reverse: GGGGTGTTGAAGGTCTCAAA | ||
| Cxcl13 | Forward: CGACTTTGAAAGGTTGCTTGTA | 219 |
| Reverse: ACACTGGATGAATAGGAAACGT | ||
| Ccl3 | Forward: AGAGGCAGCGAGTACCAGTCC | 184 |
| Reverse: CATAGGAGAAGCAGCAGGCAGTC | ||
| Cxcl11 | Forward: GCTGTGACAAAGTTGAAGTGAT | 102 |
| Reverse: GTATTGTCTGCATTATGAGGCG | ||
| Cd68 | Forward: GCTGTGACAAAGTTGAAGTGAT | 200 |
| Reverse: GGCTGGTAGGTTGATTGTCGTCTC | ||
| Mettl14 | Forward: TGAGAGGCTTAGACCGAAGTCACC | 104 |
| Reverse: GACCACGGCCAGCAGATGTTC | ||
| Pcdh18 | Forward: CCAGAGTCACAACAGTCACCAGTC | 109 |
| Reverse: CTCAACAGCAGGAGTGGCATGG | ||
| Nap1l4 | Forward: GGCACAGTGAGAATGAGGAGGATG | 163 |
| Reverse: ACCAGTTCGCTAAGCATGTCCAC | ||
| LOC691141 | Forward: AGGCGGTCCAGCTCCTACAAG | 121 |
| Reverse: ACACCATACTGACAGCGGAAGTTG | ||
| Pdk4 | Forward: GGCCACGGTCGAGCATCAAG | 168 |
| Reverse: GGCGTTGGAGCAGTGGAGTATG | ||
| Slco2a1 | Forward: ATCCTATTCGCCATCGCTGTGTTC | 94 |
| Reverse: TGTCCACTCTGCCGTAGTCCAC | ||
| Fgf21 | Forward: TCTCCTGCTGCCTGTCTTCCTG | 129 |
| Reverse: TCGGTGTCCTGGTCGTCATCTG | ||
| Zbtb16 | Forward: CACCAGCAAGATGTTTGAGATC | 239 |
| Reverse: CTGTGTCATTGTCATCAGATGC | ||
| Slc10a6 | Forward: TCCTTCTCTGCTGAGTACCTGGTC | 102 |
| Reverse: TGCCTGATATGCTGCGACAATGAG | ||
| 102552154 | Forward: ATCGCTAGTTATGCAGGCATGGAG | 124 |
| Reverse: CAGCCAATGAGTTGCACTTGAACC | ||
| 108351753 | Forward: CAGTGGTTGTGAGCTGTCTGAGG | 148 |
| Reverse: ACGCAAGCATGGTAGGTGTTGG | ||
| 102546487 | Forward: AGCCTCAGCCTTAAGCATGTGTTC | 85 |
| Reverse: CCTTGTCTCCTCGGTTGGATGTG | ||
| 108349645 | Forward: AGGAGCACGGAGATAGCAACTGG | 108 |
| Reverse: CGGTGAGTACAGTGCTTGCCATAC | ||
| 108352578 | Forward: CGCGAACACCGGAACTCTTAGATC | 125 |
| Reverse: TGCCACTTGTGCTCAGCTTCTTC |