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. 2020 Nov 23:lmaa099. doi: 10.1093/labmed/lmaa099

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Published by Oxford University Press on behalf of American Society for Clinical Pathology 2020.

This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

This work is written by (a) US Government employee(s) and is in the public domain in the US.

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Left: One of the prepared aliquots of transport media. Note the pink color because of the phenol red. In the presence of acidity (perhaps because of bacterial growth), this color would show yellowing, and thus this coloration remains a quality control tool. This image was shown in an abstract presentation of these data.¹ Right: One aliquot of prepared VTM was generously inoculated with a large quantity of Escherichia coli and then incubated in the CO₂ incubator at 37ºC for 24 hours. Because of bacterial growth, the VTM has a yellow appearance.