Figure 1.

XIST expression was up-regulated in NSCLC tissues and cell lines, and correlated with the radioresistance. (a) Expression of XIST in NSCLC tissues and adjacent lung tissues was examined by qRT-PCR. (b) Expression of XIST was evaluated by qRT-PCR in NSCLC cell lines and normal bronchial epithelial cell line (16HBE). (c) H838 and A549 cells were transfected with si-XIST-1 and si-XIST-2, and transfection efficiency was detected by qRT-PCR. (d) Representative images of colony formation assays (under 4 Gy irradiation). (e) Colony formation assay was used to measure colony survival rate 2 weeks after H838 and A549 cells transfected with si-XIST or si-con were exposed to the indicated single doses of irradiation (0, 2, 4, 6, or 8 Gy). (f) CCK-8 assay was performed to determine cell proliferation of H838 and A549 cells. (g) and (h) The apoptosis of H838 and A549 cells was evaluated by flow cytometry.

*P < 0.05, **P < 0.01 and ***P < 0.001. si-con vs si-XIST.