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. 2021 Feb 14;35:2058738420966087. doi: 10.1177/2058738420966087

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — XIST acted as the sponge of miR-16-5p. (a) The potential binding site between XIST and miR-16-5p was predicted by bioinformatics. (b) Dual luciferase reporter assay showed that XIST could bind with miR-16-5p. (c) After transfecting H838 and A549 cells with pcDNA-NC, pcDNA-XIST, si-con and si-XIST, the expression of miR-16-5p was detected by qRT-PCR. (d) The enrichment of XIST in nucleus and cytoplasm was measured by qRT-PCR. (e) A negative correlation between the expressions of XIST and miR-16-5p was observed in NSCLC tissues.

***P < 0.001. ns, not significant.