Figure 4.

Down-regulation of miR-16-5p expression impairs the effects on NSCLC cell proliferation and radiosensitivity induced by XIST knockdown. si-con, si-XIST or si-XIST+anti-miR-16-5p was transfected into H838 and A549 cells, respectively. (a) Cell viability of NSCLC cells was measured by CCK-8 assay. (b) The survival rate of NSCLC cells was detected by colony formation assays 2 weeks after being exposed to different doses of X-ray irradiation (0, 2, 4, 6 or 8 Gy). (c) and (d) The apoptosis of H838 and A549 cells was evaluated by flow cytometry.

*P < 0.05, **P < 0.01 and ***P < 0.001, si-con vs si-XIST. #P < 0.05, ##P < 0.01 and ###P < 0.001, si-XIST vs si-XIST+anti-miR-16-5p.