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. 2021 Feb 14;35:2058738420966087. doi: 10.1177/2058738420966087

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — miR-16-5p and XIST regulated the expression of WEE1 in NSCLC cells. (a) The binding site between miR-16-5p and WEE1 was predicted by bioinformatics. (b) The expression of WEE1 mRNA was detected by qRT-PCR after the transfection of miR-con and miR-16-5p mimics into H838 and A549 cells. (c) The expression of WEE1 was detected by Western blot after the transfection of miR-con and miR-16-5p into H838 and A549 cells. (d) The correlation between miR-16-5p expression and WEE1 expression in NSCLC samples. (e) WEE1-WT-3’UTR or WEE1-MUT-3’UTR plasmid was co-transfected into H838 and A549 cells with miR-16-5p or miR-con. The luciferase activity was determined at 48 h after transfection. (f) The expression of WEE1 was detected by Western blot after transfection of si-XIST into H838 and A549 cells. (g) The correlation between XIST expression and WEE1 expression in NSCLC samples.

***P < 0.001, si-XIST vs si-XIST+anti-miR-16-5p.