Figure 1.

TS, TP, DPD and COX2 genes methylation forms (U, UM and M) for selected number of 43 CRC patients. Each 3 consecutive bands after the DNA ladder represent the gene expression before and after 3 and 6 months of FP therapy for each CRC patient: (a) the unmethylated genes primers in the left side (UTS, UTP, UDPD and UCOX2) and (b) the methylated genes primers in the right side (MTS, MTP, MDPD and MCOX2).

Abbreviations: U, unmethylated; UM, partial methylated; M, methylated genes.

DNA extracted from patients at baseline and after 3 and 6 months of FP therapy were bisulphite converted, and then amplified with the methylated and un-methylated primers of the target genes. PCR products were horizontally electrophoresed and imaged on UV-transilluminator system.