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. 2021 Feb 14;14:2516865720986231. doi: 10.1177/2516865720986231

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — DPD expression in healthy control and baseline CRC patients (a) and the change in DPD expression after 3 and 6 months of FP therapy normalised to their baseline levels (b).

^aIs a significant difference when CRC patients after 3 and 6 months of FP therapy were compared with their baseline level, P value ⩽.05.

^bIs a significant difference when CRC patients after 6 months of FP therapy were compared with their level after 3 months of FP therapy, P value ⩽.05.

Total RNA was extracted from the isolated lymphocytic cell pellets of healthy control and CRC patients at baseline and after 3 and 6 months of FP therapy. RNA was converted into cDNA, and then RT-PCR amplification was conducted with the designed DPD gene sequence.