Table 1.
Genes | Primers | Sequence | Tm |
---|---|---|---|
TS | M-Forward primer | AAGGCGCGGTCGATTAGAC | 53.2 |
Sequence ID: NG_028255.1 | M-Reverse primer | AAAACCACGAATATAACACAA AAC G | |
Region: 4784. . . .4818 | U-Forward primer | GGT TTA GAG AAG GTG TGG TTGATTAGAT | 57 |
U-Reverse primer | CACAAAACCACAAATATAACACAAAAC A | ||
TP | M-Forward primer | TTAGCGTTGCGTCGCGTT C | 53.2 |
Sequence ID: NG_011860.1 | M-Reverse primer | CCGACCAATCCCCCGATA C | |
Region: 3816. . . .3825 | U-Forward primer | TGG GAT TTTAGTGTTGTGTTGTGTTT | 53.2 |
U-Reverse primer | CCCAACCAATCCCCCAATAC | ||
DPD | M-Forward primer | GGTTGTCGTGTTTGGCGC | 52.6 |
Sequence ID: NG_008807.2 | M-Reverse primer | ATC TAC CAA TAA CAA ACC CTCCTTACG | |
Region: 132138. . .132151 | U-Forward primer | GTTGTGGTTGTTGTGTTTGGTGT | 53.5 |
U-Reverse primer | ATCTACCAATAACAAACCCTCCTTACA | ||
COX2 | M-Forward primer | TTAGATACGGCGGCGGCGGC | 60 |
Sequence ID: NG_028206.2 | M-Reverse primer | TCTTTACCCGAACGCTTCCG | |
Region: 4536..4549 | U-Forward primer | ATAGATTAGATATGGTGGTGGTGGT | 60 |
U-Reverse primer | CACAATCTTTACCCAAACACTTCCA |