Table 4.
The association of the promoter methylation of fluoropyrimidine metabolising genes with their expression level in CRC patients.
| TS promoter methylation form | % of baseline CRC patients |
|||
|---|---|---|---|---|
| Total (N = 43) (%) | TS baseline median expression level (%) |
P-value | ||
| ⩽2.98 | >2.98 | |||
| UTS | 90.7 | 42.0 | 36.0 | .793 |
| UMTS | 2.3.0 | 2.0 | 0.0 | NA |
| MTS | 7.0 | 0.0 | 6.0 | NA |
| TP promoter methylation form | Total (N = 43) (%) | TP baseline median expression level (%) |
P-Value | |
| ⩽6.92 | >6.92 | |||
| UTP | 0.0 | 0.0 | 0.0 | NA |
| UMTP | 0.0 | 0.0 | 0.0 | NA |
| MTP | 100 | 47.1 | 40.8 | NA |
| DPD promoter methylation form | Total (N = 43) (%) | DPD baseline median expression level (%) |
P-Value | |
| ⩽4.26 | >4.26 | |||
| UDPD | 0.0 | 0.0 | 0.0 | NA |
| UMDPD | 4.7 | 3.9 | 0.0 | NA |
| MDPD | 95.3 | 58.8 | 24.5 | <.001 |
| COX2 promoter methylation form | Total (N = 43) (%) | COX2 baseline median expression level (%) |
P-Value | |
| ⩽3.95 | >3.95 | |||
| UCOX2 | 4.7 | 2.0 | 2.0 | NA |
| UMCOX2 | 39.5 | 25.5 | 8.2 | .006 |
| MCOX2 | 55.8 | 35.3 | 12.2 | .002 |
Data presented as the percentage of patients at different promoter gene methylation forms (U, UM and M) and different levels of expression. The median baseline expression levels of TS, TP, DPD and COX2 in 43 CRC patients were 2.98, 6.92, 4.26 and 3.95 were. Significant P-values marked with bold italic font.
Abbreviations: U, unmethylated; UM, partial methylated; M, methylated; TS, thymidylate synthase; TP, thymidine phosphorylase; DPD, dihydropyrimidine dehydrogenase; COX2, cyclooxygenase 2; NA, not applicable.