Table 2.

Mutation efficiency of AtBRI1 gene in system leaf and transgenic M1 generation

Transgenic receptor	Modification	Target	System leaf mutation frequency of M0	Plant number	No. of M1 edited plants showing excepted phenotype	No. of M1 edited plants	Mutation frequency of M1
Pro35S::Cas9	Unmodified sgRNA	BRI1-sgRNA	(3/12) 25%	#2	N/A	0	0.00%(0/68)
				#4	N/A	0	0.00%(0/48)
				#7	N/A	0	0.00%(0/72)
	FT-sgRNA	FT-BRI1-sgRNA	(3/12) 25%	#2	N/A	3	4.35%(3/69)
				#6	N/A	4	8.33%(4/48)
				#10	N/A	8	8.79%(8/91)
ProYao::Cas9	Unmodified sgRNA	BRI1-sgRNA	(8/16)50%	#12	N/A	0	0.00%(0/44)
				#13	N/A	0	0.00%(0/60)
				#14	N/A	0	0.00%(0/64)
	FT-sgRNA	FT-BRI1-sgRNA	(3/12) 25%	#3	N/A	3	6.52%(3/46)
				#4	N/A	5	8.06%(5/62)
				#11	N/A	3	5.56%(3/54)