Figure 5.

Repression of MOS with its cascade effect and phenotypic rescue by dehydrocorydaline chloride. (A) The MOS level in oocyte groups of negative control, PATL2^WT or PATL2^Y217N cRNA microinjected were determined by western blot. (B) Illustration of mRNA microinjection and oocyte culture. (C) Fluorescence results showing the expression of GFP fused with Mos 3′-UTR. The scale bar represents 50 μm. (D) ERK1and pERK1/2 protein levels in in oocytes of negative control, PATL2^WT or PATL2^Y217N cRNA microinjected were determined by western blot. (E) Representative images of normal control oocytes and that of PATL2^Y217N cRNA injected oocytes were cultured with/without dehydrocorydaline chloride. Scale bars indicate 50 μm. (F) Quantitative analysis of Pb1 extrusion rate. Data are represented as the mean ± SD; *p < 0.05.