Fig. 5.

CIH increased Bach1 expression and CSC-like property. a The protein expression of Bach1 in lung cancer nodules from Tumor + Nor or Tumor + CIH mice by Western blot analysis. b Quantification of relative protein expression was performed by densitometric analysis, and GADPH acted as an internal control. The protein expression of Bach1, Nanog, SOX2, and OCT4 in A549 (c) or SPCA1 (d) after CIH exposure for 48 h by Western blot analysis. e Quantification of relative protein expression was performed by densitometric analysis, and GADPH acted as an internal control. All data are presented as the mean ± SEM. *P < 0.05 compared with the control group. Every experiment was repeated at least three times. CIH chronic intermittent hypoxia, CSC cancer stem cell