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. 2021 Feb 18;21:119. doi: 10.1186/s12935-021-01830-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — TEK knockdown promoted the proliferation and migration of ccRCC cells. a, b Verification of TEK-siRNA silencing efficacy on the mRNA and protein levels of ACHN cells and Caki-1 cells. c MTT assay detected the effect of TEK silencing on cell proliferation. d Immunofluorescence staining of Ki-67. e The cloning formation assay evaluated the effect of silencing TEK on cloning formation ability. f Statistical analysis of cloning formation assay. g The effect of TEK silencing on cell migration was evaluated by transwell assay. h Statistical analysis of transwell assay