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. 2021 Feb 17;40:72. doi: 10.1186/s13046-021-01868-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — MAPKAPK5-AS1 acts as a sponge of miR-154-5p in HCC cells. a Subcellular fractionation assay was applied to determine MAPKAPK5-AS1 subcellular localization. b 15 putative targets of MAPKAPK5-AS1 was predicted by applying two bioinformatics prediction tools. c qRT-PCR showed that MAPKAPK5-AS1 negatively regulated miR-154-5p expression in HCC cells. d The predicted binding sites between miR-154-5p and MAPKAPK5-AS1 and mutant sequences of the potential miR-154-5p binding site in MAPKAPK5-AS1. Additionally, the result of luciferase reporter assay is shown here. (E) Anti-Ago2 RIP assay was performed in HCCLM3 with miR-154-5p overexpression, followed by qRT-PCR to detect the level of MAPKAPK5-AS1 or miR-154-5p associated with Ago2. f The expression level of miR-154-5p in HCC tissues and adjacent non-tumor tissues. g The relativity between MAPKAPK5-AS1 and miR-154-5p in HCC tissues. *p < 0.05