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. 2021 Feb 3;11:603059. doi: 10.3389/fimmu.2020.603059

Figure 2.

Figure 2

RORγt+FoxP3+ Tregs are induced peri-weaning and are present in the adult colon lamina propria. Control mice (gray) and FoxP3iDTR mice (dark blue) were injected with tamoxifen on DOL21, diphtheria toxin on DOL24, and analyzed on DOL28: (A) absolute number of Foxp3+ CD4 T cells and (B) absolute number of T cells subsets in colon lamina propria, analyzed by two-tailed Student’s t test. (C) Experimental schematic describing labeling of Tregs with DTR using tamoxifen by injecting FoxP3iDTR mice on DOL14 (light blue) or DOL24 (purple). C57Bl6 mice were used as controls and injected with a vehicle (black) or tamoxifen on DOL24 (gray). All groups were injected with diphtheria toxin on DOL56 for deletion of labeled cells on DOL 56. (D) Absolute number of Foxp3+ or RORγt+FoxP3+ colon LP cells analyzed on DOL 63 following depletion at DOL 56, analyzed by one-way ANOVA with Dunnett’s test for multiple comparisons. n = 4 mice per group in panels (A–C) *denotes statistical significance < 0.05, ns, not significant.