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. 2021 Feb 3;11:603059. doi: 10.3389/fimmu.2020.603059

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Copyright © 2021 Knoop, McDonald, Hsieh, Tarr and Newberry

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RORγt+FoxP3+ peri-weaning Tregs include a heterogeneous population of Tregs that express GATA3, cMAF, and Tbet. (A) viSNE plots of colon CD4 T cell populations from pooled samples denoting Foxp3+ and RORγt+Foxp3+ and Foxp3− clusters. (B–F) Plots also show individual expression of Foxp3, RORt, GATA3, cMAF, and Tbet. (G–I) Plots of CD4+ T cells in the colon LP from individual experimental conditions. Vsne analysis in (A–F) are pooled from n = 3 mice per group, plots in (G–I) are representative of n = 3 mice per group.