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. Author manuscript; available in PMC: 2021 Jul 7.
Published in final edited form as: Cell Metab. 2020 Jun 8;32(1):128–143.e5. doi: 10.1016/j.cmet.2020.05.003

Figure 4. TLRs Promote Divergent Fatty Acid Synthetic Programs.

Figure 4.

(A) Total palmitic acid (16:0) and oleic acid (18:1) from quiescent BMDMs (indicated as NT) and BMDMs stimulated with TLR1/2, TLR3, TLR4, TLR7, or TLR9 agonists for either 24 or 48 h.

(B) Net synthesized palmitic acid (16:0) and oleic acid (18:1) quiescent BMDMs (NT) or BMDMs stimulated with TLR1/2, TLR3, TLR4, TLR7, or TLR9 agonists for either 24 or 48 h.

(C) Net synthesized palmitic acid (16:0) and oleic acid (18:1) from quiescent control or MyD88−/− BMDMs and BMDMs stimulated with TLR1/2, TLR3, TLR4, TLR7, or TLR9 agonists for 48 h.

(D) Net synthesized of palmitic acid (16:0) and oleic acid (18:1) from quiescent WT control or TRIF−/− BMDMs, and stimulated with TLR1/2, TLR3, TLR4, TLR7, or TLR9 agonists for 48 h.

(E) Net synthesized palmitic acid (16:0) and oleic acid (18:1) in control or IFNAR−/− BMDMs stimulated with TLR3 agonist or IFNβ for 48 h.

All isotope labeling experiments are from four biologic replicates per experimental condition. All data are presented as mean ± SEM. *p < 0.05; **p < 0.01, ***p < 0.001. Experiments shown are representative of more than three independent studies.