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. Author manuscript; available in PMC: 2021 Jul 7.
Published in final edited form as: Cell Metab. 2020 Jun 8;32(1):128–143.e5. doi: 10.1016/j.cmet.2020.05.003

Figure 5. NRF2/SREBP Axis Sets Basal and TLR Inducible Lipogenesis in Macrophages.

Figure 5.

(A) Net synthesized palmitic acid (16:0) and oleic acid (18:1) from WT control and SCAP−/− BMDMs stimulated with TLR1/2, TLR3, TLR4, TLR7, TLR9 agonists, or not treated (NT) for 48 h.

(B) Net synthesized palmitic acid (16:0) and oleic acid (18:1) from WT control and NRF2−/− BMDMs stimulated with TLR1/2, TLR3, TLR4, TLR7, TLR9 agonists, or not treated (NT) for 48 h.

(C) qPCR analysis of Fasn, Scd1, and Scd2 gene expression from WT control or NRF2−/− BMDMs stimulated with TLR1/2, TLR3, TLR4 agonists or not-treated (NT) for 24 h.

(D) MS analysis of itaconate from WT control or IRG1−/− BMDMs stimulated with TLR1/2, TLR3, TLR4, TLR7, TLR9 agonists or not treated (NT) for 24 h.

(E) Net synthesized palmitic acid (16:0) and oleic acid (18:1) in WT control or IRG1−/− BMDMs stimulated with TLR1/2, TLR3, TLR4 agonists or not-treated (NT) for 48 h.

All isotope labeling experiments are from four independent replicates per experimental condition and are representative of greater than three experiments. Gene expression studies are from three biologic replicates per experimental condition and are representative of greater than three experiments. All data are presented as mean ± SEM. *p < 0.05; **p < 0.01, ***p < 0.001.