KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| RBC lysis buffer | Sigma-Aldrich | Cat# R7757 |
| DMEM (High glucose) | Gibco | Cat# 11965 |
| DMEM (No Glucose) | ThermoFisher | Cat# 11966–025 |
| Penicillin/Striptomycin | Gibco | Cat# 15140122 |
| Sodium Pyruvate | Gibco | Cat# 11360070 |
| L-Glutamine | Gibco | Cat# A2916801 |
| HyClone Characterized FBS | GE | Cat# SH30071.03 |
| Recombinant M-CSF generated from CMG14–12 culture supernatant | Takeshita et al., 2000 | N/A |
| Pam3CSK4 | InvivoGen | Cat# tlrl-pms |
| Poly(I:C) | InvivoGen | Cat# tlrl-picw |
| LPS | InvivoGen | Cat# tlrl-smlps |
| CL307 | InvivoGen | Cat# tlrl-c307 |
| ODN1668 | InvivoGen | Cat# tlrl-1668 |
| Recombinant Mouse IFN-β1 (carrier-free) | BioLegend | Cat# 581302 |
| Recombinant Murine IFN-γ | PeproTech | Cat# 315–05 |
| Palmitic Acid (16:0) | Nu-Chek Prep | Cat# N-16-A |
| Palmitoleic Acid (16:1) | Nu-Chek Prep | Cat# U-40-A |
| Oleic Acid (18:1) | Nu-Chek Prep | Cat# U-46-A |
| Cholesterol | Sigma-Aldrich | Cat# C4951 |
| Trizol | ThermoFisher | Cat#15596-018 |
| D-Glucose (U-13C6, 99%) | Cambridge Isotope Laboratories | Cat# CLM-1396-MPT-PK |
| Low Density Lipoprotein from Human Plasma, Acetylated (AcLDL) | ThermoFisher | Cat# L35354 |
| Calcein-AM | Santa Cruz Biotech | Cat# sc-203865 |
| Methoxyamine hydrochloride | Sigma-Aldrich | Cat# 89803 |
| N-fert-Butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-Butyldimethylchlorosilane | Sigma-Aldrich | Cat# 00942 |
| Itaconic acid | Sigma-Aldrich | Cat# I29204 |
| Hoescht 33342 | ThermoFisher | Cat# 62249 |
| Endotoxin-low BSA | Sigma-Aldrich | Cat# A8806 |
| pHrodo Red S. aureus Bioparticles Conjugate | Invitrogen | Cat# A10010 |
| SCDi CAY10566 | Cayman Chemical | Cat# NC0493687 |
| Critical Commercial Assays | ||
| High-Capacity cDNA Reverse Transcription Kit | Applied Biosystems | Cat#4368813 |
| KAPA SYBR FAST qPCR Master Mix (2X) optimized for LightCycler 480 | KAPA Biosystems | Cat#KK4601 |
| Deposited Data | ||
| Raw and analyzed lipidomics data | This paper | https://www.metabolomicsworkbench.org/ (Project ID PR000914; https://doi.org/10.21228/M8JH69) |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | RRID:IMSR_JAX:000664 |
| Mouse: B6.129P2(SJL)-Myd88tm1.1Defr/J (MyD88−/−) | The Jackson Laboratory | RRID:IMSR_JAX:009088 |
| Mouse: C57BL/6J-Ticam1Lps2/J (TRIF’/_)) | The Jackson Laboratory | RRID:IMSR_JAX:005037 |
| Mouse: B6.129X1-Nfe2l2tm1Ywk/J (NRF2”/_) | The Jackson Laboratory | RRID:IMSR_JAX:017009 |
| Mouse: C57BL/6NJ-Acod1em1(IMPC)J/J (IRG1”/_) | The Jackson Laboratory | RRID:IMSR_JAX:029340 |
| Mouse: B6;129-Scaptm1Mbjg/J (SCAP’/_) | The Jackson Laboratory | RRID:IMSR_JAX:004162 |
| Mouse: B6;129S6-Srebf1tm1Mbr/J (SREBP1C’/_) | The Jackson Laboratory | RRID:IMSR_JAX:004365 |
| Mouse: SCD1/2 | Miyazaki et al., 2005 | N/A |
| Mouse: B6(Cg)-Ifnar1tm1.2Ees/J (IFNAR’/_) | The Jackson Laboratory | RRID:IMSR_JAX:028288 |
| Oligonucleotides | ||
| Murine Il1b primer: Fwd: GCCCATCCTCTGTGACTCAT Rev: AGGCCACAGGTATTTTGTCG | This paper | N/A |
| Murine Il6 primer: Fwd: AGTTGCCTTCTTGGGACTGA Rev: TCCACGATTTCCCAGAGAAC | This paper | N/A |
| Murine Nos2 primer: Fwd: CACCTTGGAGTTCACCCAGT Rev: ACCACTCGTACTTGGGATGC | This paper | N/A |
| Murine Mx1 primer: Fwd: GACCATAGGGGTCTTGACCAA Rev: AGACTTGCTCTTTCTGAAAAGCC | This paper | N/A |
| Murine Cxcl1 primer: Fwd: CTGGGATTCACCTCAAGAACATC Rev: CAGGGTCAAGGCAAGCCTC | This paper | N/A |
| Murine Cxcl5 primer: Fwd: TGCGTTGTGTTTGCTTAACCG Rev: CTTCCACCGTAGGGCACTG | This paper | N/A |
| Murine Cxcl10 primer: Fwd: CCAAGTGCTGCCGTCATTTTC Rev: GGCTCGCAGGGATGATTTCAA | This paper | N/A |
| Murine Irf1 primer: Fwd: GGCCGATACAAAGCAGGAGAA Rev: GGAGTTCATGGCACAACGGA | This paper | N/A |
| Murine Irf7 primer: Fwd: TCCAGTTGATCCGCATAAGGT Rev: CTTCCCTATTTTCCGTGGCTG | This paper | N/A |
| Murine Tnfa primer: Fwd: TGCCTATGTCTCAGCCTCTTC Rev: GAGGCCATTTGGGAACTTCT | This paper | N/A |
| Murine 36b4 primer: Fwd: CTGTGCCAGCTCAGAACACTG Rev: TGATCAGCCCGAAGGAGAAG | This paper | N/A |
| Murine Scd1 primer: Fwd: GCTCTACACCTGCCTCTTCG Rev: GCCGTGCCTTGTAAGTTCTG | This paper | N/A |
| Murine Scd2 primer: Fwd: TGGTTTCCATGGGAGCTG Rev: TTGATGTGCCAGCGGTACT | This paper | N/A |
| Murine Srebf1c primer: Fwd: GAGCCATGGATTGCACATTT Rev: GGGAAGTCACTGTCTTGGTTG | This paper | N/A |
| Murine Fasn primer: TGGGTTCTAGCCAGCAGAGT Rev: ACCACCAGAGACCGTTATGC | This paper | N/A |
| Software and Algorithms | ||
| Prism 8 | GraphPad Software | https://www.graphpad.com/ |
| Adobe Illustrator CC | Adobe | http://www.adobe.com/products/illustrator.html |
| Agilent MassHunter Software | Agilent | http://www.agilent.com/en/products/software-informatics/masshunter-suite/masshunter/masshunter-software |