FIG. 4.

Regional biochemical analyses of MSC-seeded agarose constructs. (A) Constructs were sectioned into 4 regions: AT, CT, AB, CB. (B, C) GAG and collagen content normalized by wet weight (%wet weight) after 4 and 8 weeks of culture. Mean ± standard deviation. ^#Versus 1V constructs within region; ^versus 3V constructs region; *versus CB region within media volume, p < 0.05. n = 3–4/per group. AB, annulus bottom; CB, center bottom; CT, center top; MSC, mesenchymal stem cell. AT, annulus top; GAG, glycosaminoglycan. Color images are available online.