Figure 7. Suture regeneration surgery performed at postnatal day 14 restores brain morphology in Twist1+/− mice with craniosynostosis.
(A) Representative magnetic resonance images (MRI) of WT, MUT and REG mouse brains. Hippocampus (Hipp), cortex (Ctx), corpus callosum (Cc) and thalamus (Th) are outlined by yellow or cyan dotted lines. Scale bar, 2 mm.
(B-F) Quantifications of volume of whole brain (B), cortex (C), hippocampus (D), corpus callosum (E) and thalamus (F). (WT, n=5; MUT, n=5; REG, n=5 mice).
(G-I) Representative images of Cux1+ (G), Ctip2+ (H), Tbr1+ (I) cells in somatosensory cortex. Scale bar, 50 μm.
(J-L) Quantification of Cux1+ (J), Ctip2+ (K), and Tbr1+ (L) cells in somatosensory cortex with 300 μm width (WT, n=4; MUT, n=4; REG, n=4 mice).
*P < 0.05, **P < 0.01, ***P < 0.001, NS, not significant calculated by one-way ANOVA with Tukey post hoc tests.