Fig. 7.

Effect of mtVapC20 expression on LRPPRC/SLIRP levels, and under conditions of mt‐SSU depletion. (A) mtVapC20 was induced for 3, 7 and 10 days. Cell lysates were prepared together with uninduced (U) controls. Levels of LRPPRC and SLIRP were determined by immunoblotting, and SDHA was used as loading control. Data for each different time point are representative of one experiment. (B, C) mtVapC20 was expressed for 4 days, concomitant with siRNA‐mediated depletion of ERAL1 or nontargeting (NT) control siRNA treatment. Cell lysates (B) and RNA (C) were prepared. Lysates were separated by 12% SDS/PAGE, and immunodetection revealed steady‐state levels of the proteins as indicated. β‐actin was used as loading control. RNA was subjected to northern blotting with probes to MTCO1, 16S mt‐rRNA and 12S mt‐rRNA to indicate changes in steady‐state levels. Probes to cytosolic 28S rRNA were used as loading control. Data obtained from both western and northern blots are representative of one experiment.