FIGURE 3.

Morpholino mediated knockdown of hypoxia inducible factor (HIF)‐2α delays embryogenesis. A, Relative mRNA expression as measured by qRT‐PCR. WT, wild‐type HH18 embryos. Error bars represent SEM, n = 2 biologically independent replicates. B,C, Determination of developmental age 44 hours postelectroporation with 5′‐mispair or EPAS1 targeting morpholinos as assessed by head‐ and tail morphology, B, (converted to Hamburger Hamilton (HH) stages. Number of embryos analyzed were n = 20 [5′‐mispair], n = 16 [EPAS1]) or counting somites ex ovo, C, (number of embryos analyzed were n = 17 (5′‐mispair), n = 15 [EPAS1]). Statistical significance was determined by one‐way analysis of variance (ANOVA). D,E, Relative mRNA expression of trunk, D, and cranial, E, neural crest associated genes in dissected neural tube tissue derived from the trunk axial level of embryos electroporated with 5′‐mispair or EPAS1 morpholinos, measured by qRT‐PCR 24 hours postelectroporation. Data presented as mean of n = 2 biologically independent repeats, error bars denote SEM. Statistical significance was determined by two‐sided student's t test