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. Author manuscript; available in PMC: 2021 Feb 18.
Published in final edited form as: Sci Transl Med. 2020 Jul 29;12(554):eaaw4974. doi: 10.1126/scitranslmed.aaw4974

Fig. 7. Effects of the human MVEC SASP on SMCs and targeted apoptosis of senescent MVECs.

Fig. 7.

pMVECs isolated from idiopathic PAH and control patients. (A) Experimental schematic (left), mRNA expression analysis (middle), and microscopy (right) of human PAH and control pulmonary MVEC senescence, induced by irradiation or TNFα. Experiments were repeated three times. (B) Proliferation of naïve SMCs cultured with medium preconditioned by senescent or nonsenescent control or PAH MVECs. Experiments were repeated five to seven times. (C) IL6 and CNN1 expression in SMCs from (B) assessed by PCR. Experiments were repeated three times. (D) MTS assay of MVEC viability with or without ABT263. MVECs were isolated from controls and PAH, with or without irradiation or TNFα-induced senescence; experiments were repeated three times. Data are reported as means ± SD. Statistics by two-sided t test or Kruskal-Wallis test. Relevant significant differences are indicated with a black bar and asterisk. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.