Fig 4.

Levels of mRNA from several ergosterol biosynthetic pathway genes during cultivation in YPD, both at 30 and 40°C, of the parental and pdr18Δ strains. A. Ergosterol biosynthetic pathway [adapted from KEGG (http://www.genome.jp/kegg/pathway.html)] in which the ERG genes under study are highlighted in blue; (B) relative quantification by qRT‐PCR of the mRNA levels from the indicated genes, using ACT1 as the reference gene. Error bars represent standard deviation resultant from at least two biological replicates with three technical replicates each. Asterisks indicate statistical significance (p > 0.05; t‐student test).