FIGURE 6.

(a, b) Photomicrographs showing Nissl‐stained sections for cytoarchitectonic purposes of the DMH combined with enzymatic detection of PHAL from the dorsomedial BNST (experiment BNST#5). (c) High magnification of the inset shown in b. Arrows represent varicosities, double arrowheads show short collaterals with terminal boutons. (d) Microphotograph illustrating the double immunodetection of PHAL‐labeled fibers (red) from the dorsomedial BNST and NeuN‐positive neurons (cyan) in the DMH. The VMH is devoid of PHAL fibers (experiment BNST#2). (e) High magnification of the double immunodetection of PHAL‐labeled fibers (red) and NeuN‐positive neurons (cyan) in the DMH. (f) 3D reconstruction using Imaris software of a PHAL‐positive axon (red, yellow arrowhead) from the dorsomedial BNST displaying several short collaterals in contact with neurons (grey) labeled with NeuN in the DMH. Scale bars are shown in the figure [Color figure can be viewed at wileyonlinelibrary.com]