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. 2020 Aug 3;529(5):929–956. doi: 10.1002/cne.24988

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© 2020 The Authors. The Journal of Comparative Neurology published by Wiley Periodicals LLC.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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(a, b) Photomicrographs showing Nissl‐stained sections for cytoarchitectonic purposes of the LHA combined with enzymatic detection of PHAL from the dorsomedial BNST (experiment BNST#5). (c) High magnification of the inset shown in b. Arrows represent varicosities along a PHAL‐positive axon, double arrowheads show short collaterals with terminal boutons. (d) Microphotograph illustrating the double immunodetection of PHAL‐labeled fibers (red) from the dorsomedial BNST and NeuN‐positive neurons (cyan) in the LHA (experiment BNST#2). (e) High magnification of the double immunodetection of PHAL‐labeled fibers (red) and NeuN‐positive neurons (cyan) in the LHA. (f) 3D reconstruction using Imaris software of a PHAL‐positive axon (red) from the dorsomedial BNST in contact with neurons (cyan) labeled with NeuN in the LHA. fx, fornix; LHA, lateral hypothalamic area; TU, tuberal nucleus. Scale bars are shown in the figure [Color figure can be viewed at wileyonlinelibrary.com]