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. 2020 Aug 3;529(5):929–956. doi: 10.1002/cne.24988

FIGURE 15.

FIGURE 15

(a) Photomicrograph showing double immunodetection of PHAL‐positive fibers (red) from the dorsomedial BNST and proopiomelanocortin (POMC)‐labeled neurons (green) in the ARH (experiment BNST#6). (b) High magnification of the double immunodetection of PHAL‐positive fibers (red) from the dorsomedial BNST and POMC‐labeled neurons (green) in the ARH. Arrow highlights a POMC neuron contacted by PHAL‐positive axons. (c) Microphotographs illustrating the double immunodetection of PHAL‐positive axons (red) in contact with POMC‐expressing neurons (green) in the ARH (experiment BNST#2). Inset on top in shows the orthogonal view on the vertical line and inset on the right shows the orthogonal view of the horizontal line. Red arrows indicate the contacting site between PHAL‐positive terminals and POMC neuron. (d) 3D reconstruction using Imaris software of a PHAL‐positive axon (red) from the dorsomedial BNST in contact with POMC neurons (green) in the ARH. (e) Microphotograph illustrating double immunodetection of PHAL‐positive fibers (red) from the dorsomedial BNST and tyrosine hydroxylase (TH)‐labeled neurons (green) in the ARH (experiment BNST#2). (f) Image showing Agouti‐related peptide mRNA (Agrp)‐expressing neurons labeled by in situ hybridization in the ARH (image credit: Allen Institute, experiment #72283799). (g) High magnification of the inset shown in f. ARH, arcuate nucleus of the hypothalamus; DMH, dorsomedial nucleus of the hypothalamus; LHA, lateral hypothalamic area; ME, medial eminence; VMH, ventromedial nucleus of the hypothalamus; V3, third ventricle. Scale bars are shown in the figure [Color figure can be viewed at wileyonlinelibrary.com]