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. 2020 Aug 3;529(5):929–956. doi: 10.1002/cne.24988

FIGURE 16.

FIGURE 16

(a) Experimental approach. A mix of AAV‐TREtight‐mTagBFP2‐B19G and AAV‐syn‐FLEX‐splitTVA‐EGFP‐tTA was injected at day 0 in the ARH of 12 week‐old Pomc‐Cre male mice. Seven days later, mice received injection of EnvA‐G‐deleted‐mcherry pseudotyped rabies. (b) Photomicrograph of the site of stereotactic injection of the viruses. Mcherry‐positive cells (red) were infected with EnvA‐G‐deleted‐mcherry pseudotyped rabies. (c–e) Microphotographs illustrating the distribution of neurons projecting onto POMC neurons of the ARH (mcherry, red), at several levels of the anterior bed nucleus of the stria terminalis (BNST). Mcherry‐positive cells are observed in the dorsomedial and lateral division of the BNST as wells as in the ventral division. ac, anterior commissure; ARH, arcuate nucleus of the hypothalamus; BNSTdm, dorsomedial division of the BNST; BNSTdl, dorsolateral division of the BNST; BNSTv, ventral division of the BNST; LSv, ventral part of the lateral septum; shy, septohypothalamic nucleus; VMH, ventromedial nucleus of the hypothalamus; VL, lateral ventricle; V3, third ventricle. Scale bars are shown in the figure [Color figure can be viewed at wileyonlinelibrary.com]