TABLE 1.
Strains and plasmids used in this study
| Strain/plasmid | Description | Origin/reference |
|---|---|---|
| FY23 (ACY192) | Wild‐type, MATa ura3‐52 leu2Δ1 trp1 | 51 |
| ACY324 | ΔSRP1::HIS3 [SRP1 CEN URA3 AmpR], MATα leu2 lys | 29 |
| ACY685 | ΔSRP1::HIS3 [SRP1 CEN URA3 Amp R], MATa leu2 trp1 | This study |
| ACY712 | ΔNUP2::KAN importin α‐GFP::HIS3 [SRP1 CEN URA3 Amp R], leu2 | 41 |
| ACY789 | ΔNUP2 srp1‐31 [SRP1 CEN URA3 Amp R], MATa ura3 leu2 his3 trp1 lys | 41 |
| ACY1237 | ΔCSE1::KAN [CSE1 CEN URA3 Amp R], MATa his3Δ1 leu2Δ0 | This study |
| ACY1552 | ΔNUP2::HIS3ΔNUP133::KAN [NUP2 CEN URA3 Amp R], MATa leu2 ura3 | This study |
| pRS315 (pAC3) | CEN LEU2 Amp R | 52 |
| pRS305 (pAC7) | 2μ LEU2 Amp R | 52 |
| pAC592 | Importin β, 2μ TRP1 Amp R | 29 |
| pAC855 | Importin α A3, CEN LEU2 Amp R | 29 |
| pAC856 | Importin α, CEN LEU2 Amp R | 29 |
| pAC858 | Importin α A55, CEN LEU2 Amp R | 29 |
| pAC883 | Importin α‐GFP, CEN URA3 Amp R | 29 |
| pAC890 | Importin α‐GFP A3, CEN URA3 Amp R | 29 |
| pAC891 | Importin α‐myc, CEN URA3 Amp R | 29 |
| pPS1176 (pAC958) | Cse1, 2μ LEU2 Amp R | P.A. Silver |
| pAC1215 | 1–51 Nup2‐GFP, CEN LEU2 Amp R | 41 |
| pAC1268 | Nup2 Δ50, CEN LEU2 Amp R | 41 |
| pAC1271 | Cse1‐GST, Amp R | P.A. Silver |
| pAC1298 | Nup2, CEN URA3 Amp R | 41 |
| pAC1303 | Cse1, 2μ TRP1 Amp R | 29 |
| pAC1342 | Nup2, CEN LEU2 Amp R | 41 |
| pAC1385 | Nup2, 2μ TRP1 Amp R | 29 |
| pAC1394 | Nup2‐GFP Δ50, 2μ URA3 Amp R | 41 |
| pAC1395 | Nup2‐GFP, 2μ URA3 Amp R | This study |
| pAC1880 | Importin α R44A, CEN LEU2 Amp R | This study |
| pAC1881 | Importin α R44E, CEN LEU2 Amp R | This study |
| pAC1882 | Importin α R44D, CEN LEU2 Amp R | This study |
| pAC1883 | Importin α R44K, CEN LEU2 Amp R | This study |
| pAC1884 | Importin α‐GFP R44A, CEN URA3 Amp R | This study |
| pAC1885 | Importin α‐GFP R44E, CEN URA3 Amp R | This study |
| pAC1886 | Importin α‐GFP R44D, CEN URA3 Amp R | This study |
| pAC1887 | Importin α‐GFP R44K, CEN URA3 Amp R | This study |
| pAC1892 | Cse1 D220A, 2μ LEU2 Amp R | This study |
| pAC1893 | Cse1 D220R, 2μ LEU2 Amp R | This study |
| pAC1894 | Cse1 D220K, 2μ LEU2 Amp R | This study |
| pAC1895 | Cse1 D220N, 2μ LEU2 Amp R | This study |
| pAC1896 | Cse1‐GST D220A, Amp R | This study |
| pAC1897 | Cse1‐GST D220R, Amp R | This study |
| pAC1898 | Cse1‐GST D220K, Amp R | This study |
| pAC1899 | Cse1‐GST D220N, Amp R | This study |
| pAC3536 | Cse1‐GFP, 2μ TRP1 Amp R | This study |
| pAC3537 | Cse1‐GFP D220A, 2μ TRP1 Amp R | This study |
| pAC3538 | Cse1‐GFP D220R, 2μ TRP1 Amp R | This study |
| pAC3539 | Cse1‐GFP D220K, 2μ TRP1 Amp R | This study |
| pAC3540 | Cse1‐GFP D220N, 2μ TRP1 Amp R | This study |
| pAC2283 | Nup2 K3E/R4D, CEN LEU2 Amp R | This study |
| pAC2286 | Nup2 R38A/R39A R46A/R47A, CEN LEU2 Amp R | This study |
| pAC2289 | Nup2 K3E/R4D R38A/R39A R46A/R47A, CEN LEU2 Amp R | This study |
| pAC2290 | Nup2‐GFP K3E/R4D, 2μ URA3 Amp R | This study |
| pAC2293 | Nup2‐GFP R38A/R39A R46A/R47A, 2μ URA3 Amp R | This study |
| pAC2296 | Nup2‐GFP K3E/R4D R38A/R39A R46A/R47A, 2μURA3 Amp R | This study |
| pAC1900 | Cse1 D220A, 2μ TRP1 AmpR | This study |
| pAC1903 | Cse1 D220N, 2μ TRP1 AmpR | This study |