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. 2020 Sep 6;236(4):2725–2739. doi: 10.1002/jcp.30039

Figure 6.

Figure 6

Overexpression of nuclear receptor coactivator 4 (NCOA4) promoted ferroptosis induced by tert‐butyl hydroperoxide (TBHP) in annulus fibrosus cells (AFCs) and nucleus pulposus cells (NPCs). AFCs and NPCs transfected lenti‐NC or lenti‐NCOA4 were exposed to 100 μM TBHP for 3 h. (a) Protein expression of NCOA4, LC3, P62, glutathione peroxidase 4 (GPX4), ferritin heavy chain (FTH), β‐actin of AFCs. (b–e) The ratio of protein (FTH, GPX4, LC3II, P62) of β‐actin in AFCs. (f) Protein expression of NCOA4, LC3, P62, GPX4, FTH, β‐actin of NPCs. (g–j) The ratio of protein (FTH, GPX4, LC3II, P62) of β‐actin in NPCs. (k,l) Immunofluorescence detection of protein expression of GPX4 in AFCs and NPCs. ***p < .001, **p < .01, *p < .05 (n = 3)