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. 2021 Jan 26;8:620459. doi: 10.3389/fcell.2020.620459

Figure 1.

Figure 1

Neuronal loss and gliosis in the central and peripheral retina after light damage. (A) Schematic of the RlbpCreER: stopf/f-tdTomato: RPE65450Leu mouse. (B) Cross section of DAPI-labeled retina 7 days after light damage with regions of analysis. (C–G) Immunofluorescence labeling for tdTomato (MG), Otx2, and DAPI nuclear staining of center (C,D) and peripheral areas (F,G) of undamaged and light damaged (7d LD) retinal sections. (E) Number of vertical cell rows of Otx2+ photoreceptors in the ONL of the central retina in controls and 7 days after light damage (7d LD). (H) Number of vertical cell rows of Otx2+ photoreceptors in the ONL of the peripheral retina in controls and 7 days after light damage (7d LD). (I–M/M′) Immunofluorescence labeling for tdTomato (MG) and GFAP of an entire retinal cross section (I), in the central (J/J′-K/K′) and peripheral areas (L/L′-M/M′) of undamaged and light damaged (7d LD) retinal sections. (N/N′-S/S′) Immunofluorescence labeling for tdTomato (MG), GFAP, and DAPI nuclear staining of the center retina 12 h, 1, 2, 3, and 5 days as well as 6 weeks after light damage (LD). Scale bar in B, I 100 μm, in C-S 50 μm. Significant differences are indicated: *p < 0.05, **p < 0.01, U-test, n control = 5, n LD = 7. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer; LD, light damage.