Figure 1.

TMZ treatment promote GSCs formation in culture. (A) Primary GBM cells were treated with TMZ for 48 h, and then cultured under the neurosphere condition for 7 days and photographed. Number and diameter of spheres on day 7 were quantified. Cell not treated with TMZ were used as controls. (B) Primary GBM cells treated with TMZ for 48 h were analyed by flow cytometry for CD133 (n = 3), MFI, mean fluorescence intensity. (C) Expression of stemness-related factors in primary glioma cells after TMZ stimulation was determined by qRT-PCR (n = 3). (D) Expression of stemness-related factors in primary glioma cells after TMZ stimulation was determined by western blot (n = 3). Data are represented as mean ± SEM, ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.