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. 2021 Feb 1;9:620883. doi: 10.3389/fcell.2021.620883

Figure 4.

Figure 4

TMZ promote GSCs formation by upregulating HMGB1. (A,B) qRT-PCR and western blot analysis of the expression level of stemness-related factors in primary GBM cells co-cultured with supertanant from the following cells: Ctrl siRNA; Ctrl siRNA + TMZ (300 μM); HMGB1 siRNA + TMZ (300 μM). (C) Images of subcutaneously xenograft glioma models in nude mice at day 24 after first treatment. Vivo tumor volume was measured every 3 days and the same day collected data were compared among groups. α < 0.05, β < 0.01, γ < 0.001, compared to control group; δ < 0.05, compared to TMZ group. (D) Images of glioma tumors taken from the mice of each group and the weight of the tumor is measured at day 24 after first treatment. Data are represented as mean ± SEM, *P < 0.05; **P < 0.01; ***P < 0.001.