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. Author manuscript; available in PMC: 2021 Feb 18.
Published in final edited form as: J Vis Exp. 2020 Sep 2;(163):10.3791/61515. doi: 10.3791/61515

Figure 2: ll Drosophila melanogaster life cycle stages, imaged by μ-CT.

Figure 2:

A Samples stained with iodine and imaged hydrated in water. Shown is a single 2D slice. (A) An embryo that has completed the early stages of gastrulation (asterisk). (B) A third instar larva. (C) A P7 pharate adult during metamorphosis. (D) An adult female. Various organs are highlighted: BWM, body wall muscles; Br, brain; Cd, cardia; Cr, crop; DLMs, dorsal longitudinal muscles; DVM, dorsal ventral muscles; E-AD, eye-antennal disc; Em, embryo; FB, fat bodies; FBCs, fat body cells; H, heart; Hg, hindgut; La, lamina; L, leg; Mg, midgut; OL, brain optic lobe; Ov, ovipositor; PC, pupal cuticle; SG, salivary glands; VNC, ventral nerve cord; W, wing; WD, wing disc. Scale Bars (A) = 100 μm; (B)-(D) = 500 μm. D, Dorsal; A, Anterior; L, Left. Scanning parameters: Source to Sample Distance (mm): (A, D) 36.5, (B) 48.8, (C) 40.3. Source to Camera Distance (mm): (A, D) 350, (B, C) 285. Camera Pixel Size (μm): (A-D) 11.6. Image Pixel Size (μm): (A, D) 1.2, (B) 1.9, (C) 1.7.