| 100% Ethanol |
|
|
For critical point drying |
| Bouin’s Solution |
Sigma-Aldrich |
HT10132 |
For animal fixation |
| Critical Point Dryer |
|
|
Dries samples using the critical point method; multiple options available (Balzers CPD 020 or Leica EMCPD300) |
| Dragonfly Software |
Object Research Systems |
|
For visualization and segmentation of micro-CT datasets; https://www.theobjects.com/dragonfly/index.html
|
| Heat Block |
|
|
For microfuge tubes |
| Image Analysis Workstation |
|
|
Should contain sufficient RAM and quality graphics card for 3D rendering |
| Iodine Solution (I2KI) |
Fisher Scientific |
SI86-1 |
For staining |
| Microcomputed Tomography Scanner |
Bruker |
Skyscan 1172 |
Cone-beam X-Ray geometry; detector is a Hamamatsu 10 MP camera with 11.54 μm pixel size. |
| Microcomputed Tomography Scanner Software |
Bruker |
|
For controling the scanner itself (e.g., performing flat field corrections, X-ray tube power, camera expsoure times, acquisition, etc.) |
| Minutien Pins |
Fine Science Tools |
26002-15 |
For poking hole in cuticle |
| NRecon Image Reconstruction Software |
Bruker |
|
Used to reconstruct cross-section images from 2D projection images taken with cone-beam X-Ray geometry |
| P10 pipet tips |
Genesee Scientific |
24-120 |
Sample mounting |
| Phosphate Buffered Saline |
Resarch Products International |
P32060-4000.0 |
Dilute to 1X with water before use |
| Phosphotungstic Acid Hydrate |
Sigma-Aldrich |
79690-25g |
For staining |
| Pin Holder |
Fine Science Tools |
26018-17 |
For Minutien Pins |
| Triton X-100 |
Research Products International |
111036 |
To remove waxy coating from adult flies (as 0.5% PBST) |
| X-Ray Microscope |
Zeiss |
Xradia 520 Versa |
Cone-beam X-Ray geometry featuring Fresnel zone plate objective lenses for Resoluton at a Distance (RaaD™) |
